Extracting Reads from paired end data in SAM file that map to a list of contig IDs
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4.1 years ago
yp19 ▴ 70

I have paired end data in a SAM file and I want to extract all the reads which correspond to specific contig ids. My contig ids are in a text file with one ID per line.

Output of SAM/BAM is preferred.

Thanks for your help.

bam reads sequencing • 1.7k views
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would the solution in that link also work for paired data? Thanks!

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@max_19 why wouldn't it work for paired data ?

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If one read in a pair matches the contig but the other read in that pair does not. Is there a way to include that pair even if only one read matches?

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4.1 years ago

ah, I see. Using awk, matching chromosomes RF01 or RF02

/samtools view -h S1.bam | awk -F '\t' 'function fun(C) { return C=="RF01" || C=="RF02";} /^@/ {print;next;} {if(fun($3) || fun($7)) print;}'

or using samjdk: http://lindenb.github.io/jvarkit/SamJdk.html

 java -jar dist/samjdk.jar -e 'Predicate<String> f=C->C.equals("RF01") || C.equals("RF02"); return (!record.getReadUnmappedFlag() && f.test(record.getReferenceName())) || (!record.getMateUnmappedFlag() && f.test(record.getMateReferenceName()));' in.bam
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That does what I need, thank you!

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