Question: envelope and alignment coordinates in hmmsearch result
gravatar for sanjeet00001992
2.1 years ago by
United States
sanjeet000019920 wrote:

I have run hmmsearch (using HMMER 3.2v) and got output in pfam tabular format. For domain position i got two co-ordinates (start and end) i.e envelope co-ordinates and alignment co-ordinates. I have used highly conserved sequence to build my hmm profile to search against a proteome. I will used these co-ordinates to construct phylogenetic tree and modelled the structure. Which co-ordinates correctly defines my domain hits? And according to which co-ordinates I should trimmed/extract my sequence to select my domain position. I am confused to choose between these two (envelope and alignment) co-ordinates.

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