**0**wrote:

Hi! here is my question. I use the UCSC xena browser to download TCGA data (log2 transformed) and I would like to classify my genes of interest according to their log2 FC (respect to normal tissue) and their respective p-value. To calculate log2 FC I took the mean expression value of gene X in the tumor tissue and subtracted it to the mean expression value of gene X in normal tissue. But to calculate the p value of that FC I'm not sure if it is correct to consider the p-value of the t test performed from the expression of gene X (log2 value) between normal and tumor tissue. If this is not the case, could you guide me on how to calculate this p value?.

Thanks!

Maria

**53k**• written 12 months ago by mariatbranham •

**0**