Is there a standard file format for anchored contigs?
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2.8 years ago
brogroh • 0

Hi,

Given a genome with many unordered contigs, and some external information that can be used to anchor these to chromosomes/linkage groups, is there a standard file format for specifying the linkage relationships between contigs? Downstream analyses will rely on this order, for example, window-based calculations of popgen summary statistics. For example, I can map the set of linkage markers to the reference using a short-read aligner, and determine that a certain set of contigs belong to linkage group X, and are in a particular order. Should this simply be represented in a fasta file with linkage information encoded in the header?

Thanks!

genome assembly linkage map anchor contigs contigs • 716 views
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One commonly used method is to link contigs into scaffolds by an arbitrary number of Ns - for example, 10 Ns: ACGTNNNNNNNNNNACGT.

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2.8 years ago
Malcolm.Cook ★ 1.3k

ALLMAPS: robust scaffold ordering based on multiple maps (github: ALLMAPS) project documentation includes ALLMAPS: How to use different types of genomic maps which does a good job of outlining the various formats that are be found in the wild and provides tools for inter-conversion and many other useful functions.

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