I want to identify subgroups in one of cancer dataset. Before using it, I have few questions :
1) What are minimum sample size required to run ConsensusClusterPlus . I have data of 19 samples . Shall I use it for clustering or I should go for other method (eg PCA).
2) While going through manual, I found it will take input data of expression values (normalised or unnormalised ?? ). I also have z score (from expression data) obtained from another analysis for 19 samples. Can I use z score directly and perform clustering using ConsensusClusterPlus software.
I will appreciate all suggestions.