Hi,
I have raw read count matrix of HTG EdgeSeq for two patients A and B; I have tumour of patient A cultured in one media and tumour of patient B cultured in two medias, so I have
Patient A tumour cultured on OGM media
Patient B tumour cultured on DMEM and OGM medias
How I can say if DMEM and OGM medias influence the tumour culturing or not as I don't have any replication?
I found deAna for Differential expression analysis between two sample groups but I am stopping by error
Any suggestion please?
Not two media. Same media but for obvious reason cultured independently.
How can you say that since media is identical in both cases.
I think that there are two medias - media 1: DMEM, media 2: OGM?
Sorry, DMEM and OGM are two different medias