I have raw read count matrix of HTG EdgeSeq for two patients A and B; I have tumour of patient A cultured in one media and tumour of patient B cultured in two medias, so I have
Patient A tumour cultured on OGM media
Patient B tumour cultured on DMEM and OGM medias
How I can say if DMEM and OGM medias influence the tumour culturing or not as I don't have any replication?
I found deAna for Differential expression analysis between two sample groups but I am stopping by error
Any suggestion please?