Question: How does DEXSeq calculate exon usage values
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9 months ago by
sneha108.ss0 wrote:

Hi, I am using DEXSeq to identify differential splicing events from RNA-seq data and I did not completely understand how DEXSeq calculates exon usage. It will be really helpful if someone could please explain how DEXSeq calculates exon usage values and how it differs from normalized counts.

Also when I plotted the exon usage values using the plotDEXSeq function, I noticed that the exon usage values on the graph does not correspond with the values from the DEXSeq output files. Could you please help me understand why there is a difference.

Thank you!

ADD COMMENTlink modified 9 months ago by Nicolas Rosewick8.3k • written 9 months ago by sneha108.ss0
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9 months ago by
Belgium, Brussels
Nicolas Rosewick8.3k wrote:

Here is the original paper from Simon Anders et al.

Everything is explained in it.

ADD COMMENTlink written 9 months ago by Nicolas Rosewick8.3k
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