How To Find RNA-seq data is Strand-Specific or not based on only fastq files from information available in public databases. I am looking for information because I might not know if such information is also deposited in public repositories. Right now I am interested in knowing this information about SRA/GEO dataset GSE72086?
Question: RNA-Seq:How to know RNA-seq data is Strand-Specific or not based on only fastq files available in public data repositories such as SRA?
13 months ago by
ASid • 10
ASid • 10 wrote:
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5 weeks ago by
Shicheng Guo • 8.0k
Shicheng Guo • 8.0k wrote:
the best way should be
infer_experiment.py in RSeQC.
pip install RSeQC infer_experiment.py -r knowngene.hg19.bed12 -i Aligned.sortedByCoord.out.bam
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