Hi there, Is there any software tool or database to identify the entire 5' and 3' UTR regions of bacterial gene? I am aware that eukaryotic genes are clearly annotated in Ensembl and Genbank with these details. But unfortunately I couldnt able to find this information for bacterial genes. Your help on this would be very much appreciated. Many thanks in advance.
If you don't have some transcriptional data, there's no way to define this specifically (and even then its pretty woolly).
It's easy enough to just take the preceeding n bases from the start codon of a gene though. Most regulatory elements will be within 500-1000bp upstream of most genes.
What exactly are you looking for an how specific are you trying to be?