Question: Cross-platform Assembly (PacBio & Ion Torrent)
gravatar for l.algibe
12 months ago by
l.algibe0 wrote:

I have sequenced a genome of a cyanobacterium with two different sequencing methods, PacBio RS and Ion torrent. I wanted to know if there is a tool to compare both sequences since I know that one has more sequence than another and I want to know where the missing gaps are.

Thanks very much

ADD COMMENTlink written 12 months ago by l.algibe0

The term you are looking for is a "hybrid assembly". Using that for googling will give you plenty of hits.

ADD REPLYlink written 12 months ago by WouterDeCoster42k

Thank you very much, I will try to find it like that

ADD REPLYlink written 12 months ago by l.algibe0

Use the pacbio assembly only, and if you like map Ion torrent reads to the pacbio contigs. There is an absolutely massive difference in read length and quality between the two!

If unclear : ion torrent is pretty awful

ADD REPLYlink modified 12 months ago • written 12 months ago by colindaven1.9k
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