I apologize for a rather basic question but I am confused about the terminology.
What is really the difference between:
- Number of passing filters
- Number of clusters
- Number of read pairs per lane
- Total number of reads
For instance, Hiseq 4000 should produce about 300M reads per lane. What does that mean exactly? If I sequence at PE150, does that mean the total number of expected reads should 600M? This is quite important when budgeting a project. 75,000 fragments with a 20X coverage would require 1,500,000 reads and so 0.0025 lanes of Hiseq 4000 (1,500,000/600M)?
Any help clarifying this would be highly appreciated!