Dear member, I got some interesting SRA file of Mus musculus related with my work in SRA database. Original authors have utilised Trapnel protocol for their analysis and found some interesting result but I found some lacuna, like no analysis of long non codind RNA analysis. I want to re-analyse the same data with de novo analysis via Trinity and want to add some more analysis. My question is, is it worth doing ? because already the original authors have done the work. Again, Mus musculus is having its own reference genome, so why shall i go for de novo? What is the benefit of de novo analysis over other method.
WIll the data be publishable if i re-analyse it. I know it depend on reviewers as well as editor and thus seeking suggestion to you all.
Both positive and negative comments are welcome.
Thanks in advance :)