ITSx error on HPC

Question

[Bioinfonext]

Hi,

I am running ITSx on HPC server just by calling module load

It is showing some error msg ....not sure How I can fix it?

I

TSx -i seqs.fna -o seqs-its-cleaned.fna -p /opt/apps/ITSx/1.0.11/ITSx_db/HMMs -t F --cpu 20 --preserve T --save_regions SSU,ITS1,5.8S,ITS2,LSU --minlen 150 --detailed_results "--reset T"
ITSx -- Identifies ITS sequences and extracts the ITS region
by Johan Bengtsson-Palme et al., University of Gothenburg
Version: 1.0.11
-----------------------------------------------------------------
Tue Jan 22 21:43:02 2019 : Preparing HMM database (should be quick)...
Tue Jan 22 21:43:02 2019 : Checking and handling input sequence data (should not take long)...
Tue Jan 22 21:43:13 2019 : Doing paralellised comparison to HMM database (this may take a long while)...

**Error: bad format, binary auxfiles, /opt/apps/ITSx/1.0.11/ITSx_db/HMMs/F.hmm:
binary auxfiles are in an outdated HMMER format (3/b); please hmmpress your HMM file again**
    Tue Jan 22 21:43:13 2019 : Fungi analysis of main strand finished.

**Error: bad format, binary auxfiles, /opt/apps/ITSx/1.0.11/ITSx_db/HMMs/F.hmm:
binary auxfiles are in an outdated HMMER format (3/b); please hmmpress your HMM file again**
    Tue Jan 22 21:43:13 2019 : Fungi analysis of complementary strand finished.
    Tue Jan 22 21:43:13 2019 : All processes finished.
Tue Jan 22 21:43:13 2019 : Parallel HMM-scan finished.
Tue Jan 22 21:43:13 2019 : Analysing results of HMM-scan (this might take quite some time)...
Tue Jan 22 21:43:16 2019 : Extraction finished!

[Ram]

Looks like your software may not have been built properly. Contact your sysadmin and check with them.

Answer 1

[h.mon]

The key message is this:

**Error: bad format, binary auxfiles, /opt/apps/ITSx/1.0.11/ITSx_db/HMMs/F.hmm:
binary auxfiles are in an outdated HMMER format (3/b); please hmmpress your HMM file

I don't know ITSx but I assume it depends on HMMER. The error message says the hmm profiles have been built with the wrong version of HMMER, you have to contact your at a admin and ask him to hmmpress the profiles with the correct version of HMMER.

[Bioinfonext]

Thanks, it is resolved with admin help.

[Ram]

If an answer was helpful, you should upvote it; if the answer resolved your question, you should mark it as accepted. You can accept more than one if they work.