PacBio consensus assembly quality value
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5.2 years ago

Hello

I have sequenced a BAC clone with PacBio RSII To make the assembly I used Facon through pbbioconda and for polishing I used quiver

To have an estimation of the consensus quality I remap the original bam reads file against the consensus fasta file

How to estimate a mean quality value, in other world, a consensus Phred score for the base calls of the consensus ... :-)

Thank you very much in advance Philippe

PacBio QV Assembly Falcon Quiver • 1.3k views
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