Missing transcripts (ENST numbers) in gff3 files from the ENSEMBL ftp site

Question

[goldberg.jm]

There are ENSEMBL transcripts, for example ENST00000454382, that are findable in searches of https://useast.ensembl.org/Homo_sapiens/Info/Index, but that are not present in the provided files Homo_sapiens.GRCh37.87.gff3 or Homo_sapiens.GRCh38.95.gff3 from their ftp site

ftp://ftp.ensembl.org/pub/release-95/gff3/homo_sapiens

Shouldn't the transcript be present in the big gff3 file if it is findable on the web page? Does anyone know what's up with this?

Thank you!

[goldberg.jm]

Yes indeed, a gene model for the transcript in question, ENST00000454382, is in the gff3 "patch" file available at the ENSEMBL ftp site: ftp://ftp.ensembl.org/pub/release-95/gff3/homo_sapiens/Homo_sapiens.GRCh38.95.chr_patch_hapl_scaff.gff3.gz.

Thank you Ben!

Answer 1

[Ben_Ensembl]

Hi goldberg.jm,

The transcript you gave as an example is located on an assembly exception (see 'location' in the link below): https://www.ensembl.org/Homo_sapiens/Transcript/Summary?g=ENSG00000232960;r=CHR_HSCHR6_MHC_MANN_CTG1:31705518-31709524;t=ENST00000454382

The FTP directory contains a number of GTF files which each contain sets of features depending on their location: http://ftp.ensembl.org/pub/current_gtf/homo_sapiens/

Here is a quick description of the contents of the different GTF files:

.gtf: This is the default file, it should contain the full annotation for all species except human and mouse. For human and mouse, it will contain all annotation on the primary assembly, ie excluding patch and haplotype regions. All species have one.

.chr.gtf: Contains only annotation on chromosomes, so toplevel scaffolds are excluded (patch and haplotypes are not included).

.chr_patch_hapl_scaff: Contains all annotation on all toplevel sequences, including patch and haplotype regions. It should only exist for human and mouse

Best wishes

Ben Ensembl Helpdesk

Answer 2

[goldberg.jm]

Yes indeed, a gene model for the transcript in question, ENST00000454382, is in the "patch" gff3 file available at the ENSEMBL ftp site: ftp://ftp.ensembl.org/pub/release-95/gff3/homo_sapiens/Homo_sapiens.GRCh38.95.chr_patch_hapl_scaff.gff3.gz.

Thank you Ben!

[GenoMax]

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