I am working with NGS metagenomic data. DNA isolated from gastric biopsy using the THSTI method, followed by PCR amplification of the V1-5 regions and sequencing using the 454 FLX+ approaches. Contains amplicon reads from all 41 samples. All the 39 samples were sequenced in a single multiplexed run. I need to demultiplex using the sample-specific sequencing barcodes provided in the supplementary table S5 of the following paper (https://www.nature.com/articles/s41598-017-15510-6).
I try to install sabre demultiplexing tool downloaded from github(https://github.com/najoshi/sabre). Sabre also requires Zlib, which can be obtained at http://www.zlib.net/. I also download it and install the library after that I get an error during sabre installation.
dcb@dcb-P6-2355IL:~$ cd /home/dcb/Downloads/sabre/sabre-master dcb@dcb-P6-2355IL:~/Downloads/sabre/sabre-master$ make gcc -Wall -pedantic -DVERSION=1.00 -O3 -c src/demulti_single.c src/demulti_single.c:4:10: fatal error: zlib.h: No such file or directory #include <zlib.h> ^~~~~~~~ compilation terminated. make: *** [Makefile:19: demulti_single.o] Error 1