Hi everybody, I am using david ontology functional annotation clustering. The thing is that it results very few biological function groups and in the bottom of the page there is a a square saying " 54 terms were not clustered". What does it mean? I guess that the functional annotation clustering only gives as a result the gene ontology terms that can be clustered together, and in that square of not clustered terms there are the rest of the terms, that can't be match together.
So taken this in to account, would it be correct to use the functional annotation chart, to have all the information about the gene ontology terms, then use "revigo" or something like that to group the terms? Or I only can work with the few clustered terms?
Furthermore, does anyone use a specific tool to do the gene ontology annotation or annotation clustering with few genes? or another way to study their biological function when working with small batch of genes?
Thanks in advance,