Hi all, I'm analyzing RNA-seq data, paired-end, coming from Nextera. I ran Fastqc to look at the quality and got the following report: here. So I was concerned with the amount of Nextera transposase left, so I ran trim_galore to trim it. But after trimming the report looks like this: here. The transposase is gone but now the GC content plot looks super weird. Any thoughts? Thank you.
Question: CG content changed after trimming the adaptors
6 days ago by
kintany • 10
kintany • 10 wrote:
ADD COMMENT • link •
Please log in to add an answer.
Powered by Biostar version 2.3.0
Traffic: 634 users visited in the last hour