Random SNPs simulation?
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4.0 years ago
thomasbersez ▴ 50

Hi everyone,

For an allele mining project I need to generate in silico NGS data. I have chosen to use Grinder to do so. I would like to add randomly generated SNPs to my reference genome, is any software available to do that ?

Thanks for help!

SNP • 2.0k views
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4.0 years ago
jean.elbers ★ 1.7k

You could try mutate.sh from BBTools/BBMap. mutate.sh can also produce a VCF file of the SNPs added. You can also generate random Illumina reads with the BBTool randomreads.sh https://sourceforge.net/projects/bbmap/

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4.0 years ago
Corentin ▴ 490

Hi, you can also try Simulome, it is quite customisable.

https://github.com/price0416/Simulome

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5870732/

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