With low coverage RNAseq of human tissue - ~6million reads aligned using STAR. Of the 84 samples I have a range of reads aligned to genes of between 2-7 Million reads. What is the bare minimum number of reads I can use for differential gene expression analysis? What is a sensible cut-off? Ideally I would like to retain as many samples as possible.
Question: for low coverage RNAseq how many reads assigned is the bare minimum for differential gene expression analysis
19 months ago by
senowinski • 30
senowinski • 30 wrote:
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