Hello, I'm trying to filter out some sequences from the nt database from ncbi.This is how I went with:
- 1-Download the prebuilt nt database
- 2-search entriz nucleotide database with query: "taxid3708[ORGN]"
- 3-Select "Send to File" and choose format "GI list"
- 4-Use the list of GIs from the previous step with: blastdb_aliastool -gilist sequence.txt -db nt_v5 -out nt_allergen -dbtype nucl
However, when I use this command line I get this error: BLAST Database error: No GIs were found in BLAST database
Here are some IDS from my GIlist: 1376310040 1179788179 1464315148 1551319539 1534512279
Am I retrieving the GI list the righ way? Thanks for the help.