Entering edit mode
5.1 years ago
Toshi_geo7
•
0
Hello,
I performed an amplicon targeted sequencing approach, where in I designed a primer like so . I had 60 samples in my library pool. When I did MiSeq run, I see that around 80,000 UMIs (79455.41 ± 8289) UMIs for every sample had only a single read. Around 30,000 UMIs (45474.85 ± 4508) had two reads and so forth . I was wondering if any of you have an explanation for this trend for finding only a X number of reads, X times ?
Thank you. Santosh
Column 1 - 39560 UMIs having only a single read; 5477 UMIs having 2 reads -- so forth