Hi everybody. I'm doing single-cell rna-seq analysis. My starting point are fastq files with paired end reads from NexteraXT platform. Read length is 75 bp.
I've trimmed my reads for both quality and adapter content with Trim-Galore, then I performed Fastqc-Multiqc to check if everything is ok.
I found several samples (about 60 on 350 samples) to have overrepresented sequences to various extent, not much in major part of the cases
i'd like to blast the overrepresented sequences to see what they are.
Is there a simple way to get them?
I tried to search over internet but i can't find anything...