Entering edit mode
5.1 years ago
is.birds
▴
20
Hi,
I've been using STAR (v 2.6.1b) to align RNA-seq and Poly-Ribo-seq reads to the human reference genome (hg38). The next steps in my pipeline use multi-mapping reads as part of a quality filter.
Here is an example excerpt from a STAR log file:
MULTI-MAPPING READS:
Number of reads mapped to multiple loci | 11334616
% of reads mapped to multiple loci | 17.56%
Number of reads mapped to too many loci | 797072
% of reads mapped to too many loci | 1.23%
I can't find any information about the threshold for "reads mapped to too many loci". Can anyone help with this? I'd just like to know what "too many" actually means.
Thanks!