Question: Best tool for quality trimming at 5 prime?
gravatar for crespialba
5 months ago by
crespialba10 wrote:

Which is the best tool to perform a quality trimming at 5 prime end? I have a sample with some sequences with low quality at the the beginning (as well as at the end) and I want to trim those parts with low quality.

ADD COMMENTlink modified 4 months ago by Biostar ♦♦ 20 • written 5 months ago by crespialba10

I believe nearly all quality-trim tools can do this (trimmomatic, BBduk, ... to name a few)

Question is if you should be doing so .....

ADD REPLYlink written 5 months ago by lieven.sterck5.5k

As @lieven said all tools should do the basic quality based trimming. Some may allow you to do a windowed approach (saving some additional bases). Not sure if all tools will but bbduk can.

ADD REPLYlink modified 5 months ago • written 5 months ago by genomax70k

Cross-posted on SE

ADD REPLYlink written 5 months ago by ATpoint21k
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