Hello! I am trying to use RNA-seq data to accomplish two objectives in a case vs. control study:
1 - Verify if the expression level is distinct between the case sample and the controls.
2 - We need to identify DE genes between conditions.
Unfortunately, we have only one case with the mutation, but we have six biological replicates for the control group. Is it ok to use the package edgeR, or we need to try something else?