Question: Per sequence GC content failed in Human RNA-seq Data
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gravatar for tahseen.abbas
7 days ago by
tahseen.abbas0 wrote:

Hi,

I am analyzing Human RNA-Seq data for which i initially did FastQC.Majority of my samples failed Per sequence GC content in FASTQC post trimming also (I haven't trimmed my sequences,just gave the adapter sequences while doing trimmomatic). I also checked my bad samples using FASTQ_Screen that maps reads against various species databases (mouse,rat,fungi,bacteria,rRNA) to find out if there is any contamination in my reads but i couldn't find so.

Any suggestions regarding this?

Thanks Tahseen

https://ibb.co/nmfTyj6

https://ibb.co/jTGD3j2

rna-seq • 64 views
ADD COMMENTlink modified 7 days ago by genomax64k • written 7 days ago by tahseen.abbas0

Please embed your images according to How to add images to a Biostars post

ADD REPLYlink written 7 days ago by ATpoint14k

is it a normal, ribo-depleted total RNA sample or something more exotic? (eg. amplicon, exome, or such )

ADD REPLYlink written 7 days ago by lieven.sterck4.2k
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