I am just curious to know how accurate would be the transcript quantification of the RNA-Seq reads using tools like Sailfish/Salmon without using the mapping tool like HISAT or STAR. Basically, I want to skip the mapping/aligning of the reads to it's reference genome and focus on isoform abundance study. Is this a good approach for transcript abundance study in eukaryotes?
Question: Transcript quantification using the reference transcriptome(gencode) and the raw RNA-Seq reads without mapping it to reference genome.
14 months ago by
Glory Basumata • 140
India/Guwahati/Indian Institute of Technology Guwahati
Glory Basumata • 140 wrote:
ADD COMMENT • link •
Please log in to add an answer.
Powered by Biostar version 2.3.0
Traffic: 1405 users visited in the last hour