Question

PSI BLAST Why does e-value and bit-score of same proteins change after iterations.

0

Entering edit mode

5.1 years ago

sundae306 • 0

The bit score increase, but the e-value decreased for the highest e-value from iteration 1st to 3rd

gene • 1.6k views

ADD COMMENT • link 5.0 years ago by sundae306 • 0

0

Entering edit mode

Why (or how) did you decided to go for psi-blast, in stead of eg. 'normal' blasts?

ADD REPLY • link 5.1 years ago by lieven.sterck 15k

score 0 · Answer 1 · 2019-03-17

seems like you're somewhat new to PSI-blast and having some beginner issues (cfr PSI BLAST How to tell thing about proteins while looking at the results ) .

The observed behaviour is expected for psi-blast which is due to how psi-blast works. And now the important bit of info: Do read up a bit about psi-blast (eg. https://www.ncbi.nlm.nih.gov/books/NBK2590/ ) to at least get the basics covered.