MEGAN6 and Daa files from DIAMOND

0

Entering edit mode

5.1 years ago

Dave Th ▴ 60

Hi all,

I am currently using MEGAN for my soil metagenomics. After using the latest version DIAMOND to get the DAA files, I imported these files to MEGAN along with GI mapping files (GI-Taxo, GI-KEGG, GI-SEED) downloaded from MEGAN6 website. The results show none of my sequences can be assigned to any function or taxonomy. Otherwise, the software said that my DAA files from DIAMOND are not DAA files. Did anyone face this kind of situation before?

Thank you, Dave

alignment • 4.4k views

ADD COMMENT • link 5.1 years ago by Dave Th ▴ 60

1

Entering edit mode

the daa format has been a moving target in the past - maybe the latest version is incompatible with megan6. Did you try to convert the daa with the meganizer tool?

ADD REPLY • link 5.1 years ago by Carambakaracho ★ 3.2k

1

Entering edit mode

I had a similar case I think. Have a look here: http://megan.informatik.uni-tuebingen.de/t/error-while-loading-rma-file/960

ADD REPLY • link 5.1 years ago by Juke34 8.5k

1

Entering edit mode

I already tried meganizer tool but it said Daa is not Daa file. Yesterday, I found out that the Daa file I got from DIAMOND is not actually DAA file because I used -o output.daa instead of -a output.daa. It's OK now but the number of sequences assigned to SEED is too low. It might because of my sequences or something else happened. Thank you guys.

ADD REPLY • link 5.1 years ago by Dave Th ▴ 60

Login before adding your answer.