Question: Aligning RNA-Seq short reads to a reference gene with semi global alignment
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4 weeks ago by
sr0 wrote:

Given a set of short reads from an RNA-Seq experiment and the reference sequence of a gene, my goal is to write an aligner to find out how much gene is expressed and locate the locations of its exons. Semi global alignment must be used, no end gap penalties. Could you advice me which tools could bu used or there is an aligner to use? If not I'll have to write the code by myself.

rna-seq alignment • 88 views
ADD COMMENTlink written 4 weeks ago by sr0

Is this a homework assignment? If yes, you'll learned most by writing the code yourself in my opinion.

ADD REPLYlink written 4 weeks ago by Benn6.6k

It is an assignment but instructor said that if there any ready aligners for that we can use them. We are not obligated to write a code.

ADD REPLYlink written 4 weeks ago by sr0
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