Uploading to SRA: multiple files per BioSample

Question

[jo.wolfe]

Hi,

I am trying to upload some fairly deeply sequenced data to SRA. For each species, it is basically 2 lanes of Illumina 2500 per species. The data (as formatted by my colleague who did the sequencing) is split into about 40-50 files of 400-600 Mb each, per species. Each of these files is fastq.gz.

The SRA metadata/excel file only allows 4 files per library. Can I either just add columns to this (a ridiculous amount of columns!), or is there a way to merge the 400-600 Mb files into a few larger fastq.gz files of say a few Gb each?

Thanks!

Answer 1

[swbarnes2]

You can just cat fastq.gz files together.

cat bacteria1*.fastq.gz > large_bacteria1.fastq.gz

for example.