Question: Check Conservation of a Given Protein Domain
0
gravatar for DVA
12 months ago by
DVA530
United States
DVA530 wrote:

Hello,

I am interested in a small domain of a well studied and well defined protein (Huntingtin), and would like to see how conserved this domain is across species. In particular, I would like to address two questions in a scientific (publishable) way:

How well conserved across species is this domain relative to the rest of the protein and relative to other proteins in general?

How far can I go from humans (evolutionary speaking) and find that this domain is still very similar (say >90% sequence identity) to what is found in humans?

Sorry if this is unclear, or has already been addressed. I tried to search for questions that addressed these issues but could not find anything. I have tried BLASTing the protein sequence, but that just gets me close relatives (e.g. chimps) that are almost identical. I am actually more interested in distant relatives. Any helpful program(s) or methods would be greatly appreciated.

Thanks a lot in advance for your help.

blast • 294 views
ADD COMMENTlink modified 12 months ago by Bill Pearson860 • written 12 months ago by DVA530

Have you looked at this page at NCBI: https://www.ncbi.nlm.nih.gov/cdd/?term=Huntingtin

ADD REPLYlink written 12 months ago by genomax80k
1
gravatar for Bill Pearson
12 months ago by
Bill Pearson860
Bill Pearson860 wrote:

If the region you are interested in is the 40 amino-acid huntington domain linked above, then you can answer your question by going to Pfam pfam.xfam.org) and looking up DUF3652 (or PF12372). That page has a link to the evolutionary tree for the domain [1]. If you are looking for a different region of the protein, then you might try using PSI-BLAST or JACKHMMER with the specific domain that you are interested in, to build a more sensitive model. The huntington domain is very short (40 aa), so it will be difficult to find very distant relatives, because there relatively little signal (because of the length). The Pfam domain set is probably the best you can do.

ADD COMMENTlink modified 12 months ago • written 12 months ago by Bill Pearson860
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