Question: Does strange fastQC GC content report cause a problem for WES analysis?
0
gravatar for maria2019
10 months ago by
maria201990
maria201990 wrote:

I have paired-end WES reads and looking at the forward and the reverse reads with FastQC for QC, they both show and unusual GC content distribution (Figure is attached for R1) even after adaptor contamination. Will this cause a problem for my WES analysis? If yes, how can I solve it? GC content:

fastqc wes gc content • 481 views
ADD COMMENTlink modified 10 months ago by JC9.4k • written 10 months ago by maria201990
2
gravatar for JC
10 months ago by
JC9.4k
Mexico
JC9.4k wrote:

If this is WES, you can expect a deviation for your curve, think on codon usage preference ...

ADD COMMENTlink written 10 months ago by JC9.4k
1

Aw I see your point. Thank you

ADD REPLYlink written 10 months ago by maria201990
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