Question: Does strange fastQC GC content report cause a problem for WES analysis?
0
gravatar for maria2019
25 days ago by
maria201910
maria201910 wrote:

I have paired-end WES reads and looking at the forward and the reverse reads with FastQC for QC, they both show and unusual GC content distribution (Figure is attached for R1) even after adaptor contamination. Will this cause a problem for my WES analysis? If yes, how can I solve it? GC content:

fastqc wes gc content • 100 views
ADD COMMENTlink modified 24 days ago by JC7.7k • written 25 days ago by maria201910
2
gravatar for JC
24 days ago by
JC7.7k
Mexico
JC7.7k wrote:

If this is WES, you can expect a deviation for your curve, think on codon usage preference ...

ADD COMMENTlink written 24 days ago by JC7.7k
1

Aw I see your point. Thank you

ADD REPLYlink written 24 days ago by maria201910
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