Does strange fastQC GC content report cause a problem for WES analysis?
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2.1 years ago
maria2019 ▴ 130

I have paired-end WES reads and looking at the forward and the reverse reads with FastQC for QC, they both show and unusual GC content distribution (Figure is attached for R1) even after adaptor contamination. Will this cause a problem for my WES analysis? If yes, how can I solve it? GC content:

fastQC WES GC content • 964 views
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2.1 years ago
JC 12k

If this is WES, you can expect a deviation for your curve, think on codon usage preference ...

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Aw I see your point. Thank you

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