Question: finding new peptides from transcriptom?
0
gravatar for alirezaei3333
11 months ago by
alirezaei33330 wrote:

i want to explain whole my work. i have transcriptome of scorpion. we need to find the peptides with 12 length and cystein rich. i dont familiar with any bioinformatic program. anyway, would you please to obtain this peptide, which step should be done?

sequencing rna-seq alignment • 222 views
ADD COMMENTlink written 11 months ago by alirezaei33330
1
gravatar for genomax
11 months ago by
genomax78k
United States
genomax78k wrote:

Based on your previous posts, it is not completely clear what data you have.

You have "transcriptome of a scorpion" but

To address the original question: If you know the pattern that you are looking for then you can use a program like fuzzpro from EMBOSS, (link provided for reference you will need to install EMBOSS locally if you have a large amount of data to process). There are several other programs in EMBOSS package that can be used for motif searches.

If you truly have no bioinformatics experience and can't use the command line then you are going to need help of type that would be hard to provide via an online forum. You should try to collaborate with a local bioinformatician who can assist you with this analysis.

ADD COMMENTlink modified 11 months ago • written 11 months ago by genomax78k

i obtain via Illumina Next Generation Sequencing then i isolatated favor sequnces with python and now i am looking for a blast tools

ADD REPLYlink written 11 months ago by alirezaei33330
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