Is There An Easy Way To Manually (Or Batch) Convert Fasta To Csv?

Question: Is There An Easy Way To Manually (Or Batch) Convert Fasta To Csv?

9.6 years ago by

Blunders • 1.1k

Blunders • 1.1k wrote:

SOLVED: Reading Fasta files into a custom database and either need to write a converter myself, or find one to export the data to CSV. Manually doing the conversion via an free application would be okay, a batch conversion would be better. It would be much better if the options run on Windows XP, but if not OSX/Linux, though all options must be be local, meaning I'm not going to upload the file to the an external system. The process to from install to output needs to be 5-10 to start, and then 10-20 seconds going forward.

Thanks, and if you have any questions, let me know.

fasta conversion • 8.6k views

ADD COMMENT • link •

modified 9.6 years ago by Radu • 50 • written 9.6 years ago by Blunders • 1.1k

What have you tried so far ?

ADD REPLY • link written 9.6 years ago by Pierre Lindenbaum ♦ 129k

@Pierre Lindenbaum: Ended up just using Regex in TextPad. Problem is solved. Thanks!

ADD REPLY • link written 9.6 years ago by Blunders • 1.1k

9.6 years ago by

Aleksandr Levchuk ♦ 3.2k

United States

Aleksandr Levchuk ♦ 3.2k wrote:

One-line solution

ruby -e 'first_line = true; while line = STDIN.gets; line.chomp!; if line =~ /^>/; puts unless first_line; print line[1..-1]; print ","; else; print line; end; first_line = false; end; puts' < s001.fasta

Simple script

Here is a Ruby script that does this:

#!/usr/bin/ruby

first_line = true

while line = STDIN.gets
  line.chomp!

  if line =~ /^>/
    puts unless first_line
    print line[1..-1]
    print ","  # <-- Change this to "\t" and it's a convert-fasta-to-tab
  else
    print line
  end

  first_line = false
end
puts

Just save it to a file.
Name the file convert-fasta-to-csv
to make it executable, run chmod +x ./convert-fasta-to-csv

Usage

./convert-fasta-to-csv < f001.fasta > f001.fasta.csv

To do it in batch run all .fasta files in current folder:

for i in *.fasta; do ./convert-fasta-to-csv < $i > $i.csv; done

System Requirements

You probably already have Ruby but it may not always be installed by default.

To install it on Ubuntu or Debian run: sudo apt-get install ruby
On RedHat or CentOS, run: sudo yum install ruby
On Windows, install from http://rubyinstaller.org/
On Mac, you already have it (it's a part of the operating system).

ADD COMMENT • link modified 10 months ago by RamRS ♦ 27k • written 9.6 years ago by Aleksandr Levchuk ♦ 3.2k

I use it for systems administration and sometimes for Bioinformatics research. It very elegant and easy to read. Has all the power of Perl and Python. Don't use Ruby (Prel and Python alike) for all of your Bioinformatcs because there is R. The R language has the best set of high-quality and high-performance Bioinformatics libraries (BioConductor). R's vector driven environment is a much better chose for Bioinformatcs data analysis. Downside: R scripts that import libraries do take a long time to startup, so converters like this one are best in Ruby.

ADD REPLY • link written 9.6 years ago by Aleksandr Levchuk ♦ 3.2k

Instead of comma-separated, I usually do tab-separated. To do that, simply replace print "," to print "\t". And rename the script to convert-fasta-to-tab :)

ADD REPLY • link written 9.6 years ago by Aleksandr Levchuk ♦ 3.2k

@Aleksandr Levchuk: Just wondering, what sort of functions do you use Ruby for? Meaning normally people use Perl or Python, but I was thinking about using Ruby, but there's so little BioXYZ source code.

ADD REPLY • link written 9.6 years ago by Blunders • 1.1k

9.6 years ago by

Mary ♦ 11k

Boston MA area

Mary ♦ 11k wrote:

I always thought the Scriptome project was a really cool idea, but it doesn't seem to have been updated in a while.

However, there might be some nuggets you could glean from this about how to structure a little script:

http://sysbio.harvard.edu/csb/resources/computational/scriptome/Windows/Tools/Change.html

ADD COMMENT • link written 9.6 years ago by Mary ♦ 11k

@Mary: Yes, that is cool, since the Perl is there there too. Just used Regex in TextPad to do it, so selecting you as an answer, since I was also wondering if a web service like that was around and how it was doing. Thanks!

ADD REPLY • link written 9.6 years ago by Blunders • 1.1k

-1 the perl code fails, it gives syntax error at -e line 1, near "="

ADD REPLY • link written 9.6 years ago by Aleksandr Levchuk ♦ 3.2k

@Aleksandr Levchuk: Just wondering, since I may try to use that code at some point, what version of Perl are you running, or do you know why there's an error?

ADD REPLY • link written 9.6 years ago by Blunders • 1.1k

@blunders I tested on v5.8 and v5.10 (those are default on Debain 4, 5, 6, and Ubuntu 10.10). Most likely, the Perl code was written for an earlier version and the interpreter is not backwards compatible.

ADD REPLY • link written 9.6 years ago by Aleksandr Levchuk ♦ 3.2k

@Aleksandr Levchuk: Wow, really -- what's the point then, meaning that mean all the BioXYZ code that's in use either needs to use an old Perl engine, or the code base needs to be updated, right?

ADD REPLY • link written 9.6 years ago by Blunders • 1.1k

@blunders Yes, I would not mark this as a good answer because people with the same question who visit BioStar (by Googling, etc...) will run into a trap.

ADD REPLY • link written 9.6 years ago by Aleksandr Levchuk ♦ 3.2k

9.6 years ago by

Aleksandr Levchuk ♦ 3.2k

United States

Aleksandr Levchuk ♦ 3.2k wrote:

Use your text editor's regexp search-and-replace feature.

Vim

In Vim, press Esc (normal mode) and paste the following sequence of commands:

:0,$s/>\(.*\)\n/>\1,/
:0,$s/\(.*\)\n\([^>]\)/\1\2/
:0,$s/^>//

And press Enter.

One-line Solution

input_file=001.fasta; vim -c '0,$s/>\(.*\)\n/>\1,/' -c '0,$s/\(.*\)\n\([^>]\)/\1\2/' -c 'w! alex-tmp.fasta.csv' -c 'q!'  $input_file; mv alex-tmp.fasta.csv $input_file.csv

ADD COMMENT • link modified 10 months ago by RamRS ♦ 27k • written 9.6 years ago by Aleksandr Levchuk ♦ 3.2k

9.6 years ago by

Alastair Kerr ♦ 5.2k

Manchester/UK/Cancer Biomarker Centre at CRUK-MI

Alastair Kerr ♦ 5.2k wrote:

Have a look at BioSql and the howto on bioperl for Open Biological Data Access for which it is a subset of. It does take a bit of figuring out, but once you do the biogetseq.pl script is very useful and can load both local and remote data sources.

ADD COMMENT • link written 9.6 years ago by Alastair Kerr ♦ 5.2k

@Alastair Kerr: Thanks, I'll take a look at it, since Perl currently appears to be the target language of choice within the company I'm at.

ADD REPLY • link written 9.6 years ago by Blunders • 1.1k

9.5 years ago by

Tim Rayner • 20

Cambridge

Tim Rayner • 20 wrote:

In the spirit of other one-line solutions, here's the (almost) inevitable perl version:

perl -e '$/=">"; while(<STDIN>) { next if length == 1; @x=split /\n/; printf "$x[0],$x[1]\n" } ' < your_sequence_file.fasta

I imagine it could be shorter still...

ADD COMMENT • link modified 10 months ago by RamRS ♦ 27k • written 9.5 years ago by Tim Rayner • 20

Can any kind coder tweak this for me so the output is to a file? Thanks <3

ADD REPLY • link written 8.1 years ago by poobearspam • 0

9.0 years ago by

Radu • 50

Radu • 50 wrote:

Late to the party but here is my implementation in C#:

using System;
using System.Collections.Generic;
using System.Text;
using System.IO;

namespace ConvertFASTA
{
    class fasta
    {
        static void Main(string[] args)
        {
            StreamWriter writer = new StreamWriter(args[1]);

            using (StreamReader reader = new StreamReader(args[0]))
            {
                string temp= "", line= "";
                bool firstLine = true;

                while ((line = reader.ReadLine()) != null)
                {
                    line = line.Replace("|", ",");
                    if (line.Substring(0, 1) != ">")
                    {
                        temp = line;
                    }
                    else
                    {
                        if (firstLine) temp = line + ",";
                        else temp = "\n" + line + ",";
                    }

                    firstLine = false;
                    writer.Write(temp,true);
                }
            }

            writer.Dispose();
            writer.Close();
        }
    }
}

Compile and use like this

fasta.exe input.fa output

ADD COMMENT • link modified 10 months ago by RamRS ♦ 27k • written 9.0 years ago by Radu • 50

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One-line solution

Simple script

Usage

System Requirements

Vim

One-line Solution

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