a problem while using fastq-dump to split SRR files
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5.1 years ago
zhangdengwei ▴ 210

Hi, I encountered the following errors when I used fastq-dump to split the SRR files, and the problem persisted after tried several times. The version of my fastq-dump is 2.8.2. Could you give me some advice, thanks for your help!!!

2019-04-02T01:16:11 fastq-dump.2.8.2 err: unknown while writing file within file system module - unknown system error errno='Disk quota exceeded(122)'
=============================================================
An error occurred during processing.
A report was generated into the file '/home/zhangdengwei/ncbi_error_report.xml'.
If the problem persists, you may consider sending the file
to 'sra@ncbi.nlm.nih.gov' for assistance.
=============================================================
software error • 2.5k views
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Please go into the directory where the generated fastq files are located and show the output of df -h . Also show the full command line please. Also check if the tool placed a large cache file somewhere on your disk which it did not removed after the error occurred. You can disable this using vdb-condig -idisabling the cache option. Did you use the tool to download a file or did it run on a .sra directly?

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looks like you are out of space in your working $HOME space.

To check the usage / amount of space you have, you can may be you can use;

quota --local-only

To check how much you have used try:

du -h
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Thanks! But I am sure the space is enough, now I am thinking about whether the reads generated by Illumina MiSeq is the point, since MiSeq reagents enable up to 15 Gb of output with 25 million sequencing reads and 2 × 300 bp read lengths. But I haven't found a way to address the problem yet.

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Search for the accession number you are interested in at EBI-ENA and just download fastq files directly.

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