Incomplete peaks in "Per sequence quality scores"
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3.5 years ago
kehoe ▴ 40

Hi!

I am using FastQC from Babraham Bioinformatics to analyze Illumina RNAseq output (fastq.gz).

In the "Per sequence quality scores" of my data I have what looks like incomplete peaks. My samples neither represent the examples given on their site for good or bad data (see examples below) so I am unsure how to interpret them (see samples below).

example_bad: https://ibb.co/q7vZqWn example_good: https://ibb.co/31QP5j9 mySample8_R1: https://ibb.co/vxzprCQ mySample8_R2: https://ibb.co/RSz4rxc

All my data have similar shaped incomplete peaks.

Any insight would be greatly appreciated as this is a new topic for me. Thank you in advance!

fastQC report fastqc • 627 views
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