Question: Incomplete peaks in "Per sequence quality scores"
0
gravatar for kehoe
4 months ago by
kehoe0
kehoe0 wrote:

Hi!

I am using FastQC from Babraham Bioinformatics to analyze Illumina RNAseq output (fastq.gz).

In the "Per sequence quality scores" of my data I have what looks like incomplete peaks. My samples neither represent the examples given on their site for good or bad data (see examples below) so I am unsure how to interpret them (see samples below).

example_bad: https://ibb.co/q7vZqWn example_good: https://ibb.co/31QP5j9 mySample8_R1: https://ibb.co/vxzprCQ mySample8_R2: https://ibb.co/RSz4rxc

All my data have similar shaped incomplete peaks.

Any insight would be greatly appreciated as this is a new topic for me. Thank you in advance!

fastqc fastqc report • 148 views
ADD COMMENTlink written 4 months ago by kehoe0
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1090 users visited in the last hour