Hi all, I have a basic question regarding the differential gene expression analysis (DESeq2) between the two conditions (say 1 and 2). If I have 3 samples for condition 1 and 60 samples for condition 2, would it be fine to do differential gene expression analysis between the conditions 1 and 2? Or, do I need to randomly select fewer samples from condition 2 to have a more "balanced" analysis? Are there any statistical problems associated with this? If I need to select fewer samples, then how many samples of condition 2 could be selected for the analysis?
Thanks in advance for any suggestions. I really appreciate your help.