annotate gene feature
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2.1 years ago

Hi,

I have a multi-fasta file with >15k DNA sequences. I wish to mark, UTR's, start, stop features. I tried expasy translate tool and it gives translated sequence with different possible ORFs. My objective is to find, how many sequences are full length. Kindly suggest.

Thanks.

gene feature annotation UTR • 582 views
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Is this for genome annotation? If so, there are many resources for structural annotation of a genome on biostars to search for.

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2.1 years ago

It is for multiple purpose. This is basically a isoseq data. So need to confirm how many of them are full length transcripts.

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Perhaps you could try

Tool: pinfish: tools to annotate genomes using long read transcriptomics data pinfish: tools to annotate genomes using long read transcriptomics data

and maybe also Transcriptome Annotation by Modular Algorithms (for Iso-Seq data) https://github.com/GenomeRIK/tama

This tool from EMBOSS might also be useful http://emboss.sourceforge.net/apps/release/6.3/emboss/apps/getorf.html

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