I have problems using psiblast this being my command line : psiblast -subject test.fa -in_msa aligment_test.fa -out_ascii_pssm pssm_test1.txt -ignore_msa_master
I get this error message whenever I have more that 4 sequences : BLAST options error: Repeated Seq-IDs detected in multiple sequence alignment file, please ensure all Seq-IDs are unique before proceeding. I am 100% sure I have no repeats and try also adding false sequences to my alignment to check that it would always crash when I have more that 4 sequences.
Do you have any idea of how I could proceed to get PSSMs from multiple alignments ?
Thank you very much in advance for your help, have a nice day