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5.0 years ago
haddadfatiha62
•
0
Hi, I am working on Metagenome analysis using Mothur. I am very naive to this tool… and processing the data with example data-sets as mentioned in the tutorial FROM MiSeq SOP Everything was going fine as mentioned in the tutorial until I got stuck at chimera.vsearch command.
Using ReadLine,Boost,HDF5
mothur v.1.41.0
Last updated: 04/25/2019
mothur > chimera.vsearch(fasta=stability.trim.contigs.good.unique.good.filter.unique.precluster.fasta, count=stability.trim.contigs.good.unique.good.filter.unique.precluster.count_table, dereplicate=t)
Using 64 processors.
Checking sequences from stability.trim.contigs.good.unique.good.filter.unique.precluster.fasta …
[ERROR]: M00967_43_000000000-A3JHG_1_1114_15978_19655 is not in your count table. Please correct.
Segmentation fault (core dumped)
can you help me please to knew where i went wrong!!! thank you
There was no error/warnings before you encountered this one? Did all outputs match what is shown in the tutorial? Are you sure you did not skip any steps?
Segmentation fault (core dumped)generally relates to an issue with memory / RAMM00967_43_000000000-A3JHG_1_1114_15978_19655 is not in your count table. Please correct.Surely this would be the place to start.
NO, i didn't have any problem before this command , I have the same results like tutorial but when I run chemira.vsearch i got error ,i tried to update mothur tool but nothing change