VisualMSI for MSI Calculation using NGS/WXS
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3.2 years ago
sruthi ▴ 30

Hi community. There is another tool "VisualMSI" for MSI calculation and its installation steps are here When I run the command:

visualmsi -i tumor.sorted.bam -r hg19.fasta -t targets/msi.tsv


it says visualmsi command not found.Could anyone tell me how to troubleshoot it?

TIA

next-gen sequencing • 1.2k views
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3.2 years ago

# Install

sudo make install

\$ sudo make install install visualmsi /usr/local/bin/visualmsi Installed.

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Hey Ranan, Thanks will do it. I had saved binary earlier.

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If you have saved then binary then do this:

sudo cp visualmsi /usr/local/bin

Then Execute from anywhere.

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Can bed file be given instead of tsv?

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Please go through the Target file generation: BED convert to tsv.

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Okay. Thanks will check it out. The tsv file given in the target folder is based on hg19 coordination, what must I do to get a target file based on hg38 coordination? Is it enough to convert hg38.bed to tsv?

TIA

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It is always better to use one version. Either hg19 or GRCh38. If you have hg38 then convert it to hg19 coordination system.

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Agreed. But I have been using hg38 for all my analysis, hence. Now, how do I convert hg19 coordination to hg38?

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And when the value is 0 for "entropy of tumor data", "supporting reads of tumor data" and "quality control:failed", does it mean, the sample is MSS/ No microsatellites or is something wrong?

TIA

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and yes ... you have to convert hg19 to hg38.

Something wrong. It should not be like that.

Try Using other tool:

https://omictools.com/microsatellite-instability-detection-category

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Thanks Ratan. I have read about all the tools and most of them need matched normal except mSINGS, MSIsensor. For msiNGS: I need to create baseline file with 10-20 normal samples, which I do not have access to. In MSIsensor: I'm getting 0 even after setting -c to maximum and minimum numbers.

Coming back to conversion of hg19 to hg38. What tool should I use for conversion. Tools in UCSC?

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Another doubt, I have bed file for hg38. How do I convert it to tsv? I do not understand the conversion steps mentioned here

Could you please explain it to me? TIA

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It is already well explained in the file.

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Hi, README.md file clearly says "higher the EMD, more chances of MSI locus to be instable", and EMD is evaluated against a normal sample. Then how does the algorithm work for case-only mode?

Could anyone just explain this? TIA