Hello I want to find the exact number of my genes exons. I have 2 genes: NKX2-5 and GATA4 I know NKX2-5 has 2 exons and GATA4 has 7 exons which 6 of them are in the codding sequence. but these exons number is different with NCBI, Gene, Exon count! please let me know why? for example, for in NCBI, Gene, Exon count, wrote 3 exons for NKX2-5 and 11 exons for GATA4
Refseq is the annotation of transcripts, not genes. The gene NKX2-5 has 4 refseq transcripts associated with it: NM_001166175, NM_001166176, NM_004387, and XM_017009071. Each transcript has 2 exons, but they are not the same exons in each case. If there are three exons, A, B and C, then NM_001166175, NM_001166176 and NM_004387 consist of exon A and various different versions of exon C, while XM_017009071 consists of exon A and exon B.