Question: Mirna Prediction from mirbase database
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bioz • 10 wrote:
Hai Stars, I want to identify known and conserved miRNAs from my data. For that i trimmed my reads (fastq Paired end) based on the length: 16bp minimum and maximum of 36 bp. For conserved miRNA identification i downloaded mirbase matured miRNA and i did homology search. But i didn't get any results because mirbase data base contains "U" base and which is not in my read.How to alignment when reference has "U" base instead of "T"
I also tried to change "U" to "T" from reference but i dint get any alignment percentage.Which aligner i can trust ?