I am working on
Trinity for de-novo assembly of my data but stuck at differential gene expression step (screenshot). I have 3 sample files and followed the steps as mentioned in the trinity tutorial but somehow getting this error in
Error, cmd: R --vanilla -q < matrix.counts.matrix.align_estimate_rsem_P3_vs_align_estimate_rsem_unP3.align_estimate_rsem_unP3.vs.align_estimate_rsem_unP3. EdgeR.Rscript died with ret (32512) at /app/setup/trinityrnaseq-2.2.0/analysis/diffenetialexpression/run_DE_analysis.pl line 712. WARNING: This EdgeR comparison failed...
Plz, guide me.
Best Regards, Sakshi