Hello All,
First time poster here. Our lab would like to start doing some scRNAseq work in our lab, we have no clue about hardware requirements. We have looked at some workstations, and below is our potential setup. If anyone could comment on the setup as far as what it can handle in the context of scRNAseq in mouse and human samples, such as size of each sample, number of samples, time it takes for processing differential expression analysis, etc.. we would really appreciate it.
Hardware Specs:
Dell Precision 7920 Tower
Intel Xeon Gold 6130 2.1GHz, 3.7GHz Turbo, 16C, 10.4GT/s 3UPI, 22MB Cache, HT (125W) DDR4-2666
Windows 10 Pro for Workstations (4 Cores Plus) Multi - English, French, Spanish
NVIDIA® Quadro® P2000, 5GB, 4 DP (7X20T)
128GB 8x16GB DDR4 2666MHz RDIMM ECC
Intel Dual Band Wireless AC 8265 (802.11ac) 2x2 + Bluetooth module
Operating System (Boot) Drive: SATA/SAS Hard Drive/Solid State Drive
Hard Drive Controller: Integrated Intel AHCI SATA chipset controller (8x 6.0Gb/s), SW RAID 0,1,5,10
1st Hard Drive: 2.5" 512GB SATA Class 20 Solid State Drive
2nd Hard Drive: 3.5" 4TB 5400rpm SATA Hard Drive
RAID for HDD/SSD & Front PCIe NVMe SSDs: No RAID
Other specs I have no a clue what use they're for, but they're part of the customization options on Dell's website:
Teradici Remote Workstation Access Host Card: No Remote Access Host Card
Network Cards: No Add-In Network Card (Integrated NIC only)
PCIe I/O Cards: Not Selected in this Configuration
Serial Port/PS2 Adapter: None
Many Thanks! YL
not sure whether you have to spend any money a GPU card ? I don't think it's a advantage (let alone a necessity) for this kind of analyses.
Thanks for the comment. Yes, from what I gathered searching for RNAseq information online lately it seems that GPU doesn't really matter, this information was just part of the default setting on the Dell computer we looked at.
Hi genomax, thanks for taking the time to answer my question.
For the hard drive I only considered SSD for the operating system and software. Perhaps I should choose SAS HDD or SSD for the second hard drive as well? Or is it the rpm that matters? I actual have no clue how the type of secondary storage plays into RNAseq data analysis.
Regarding the operating system, My lab wants to purchase SeqGeq as well from FlowJo, which is only available for windows or mac. As many other software we use are compatible with windows or mac, we would like to use a windows or a mac. My thinking was that I may be able to dual boot windows and linux, or use the windows subsystem for linux. In essence, we'd like to have a more versatile workstation not too constrained by operating system. Perhaps many software are indeed available in linux to substitute what we currently use, we'd like to minimize the learning curve.
Regarding sample size, right now we have 2 sample sets, one has 2 samples, the other has 4 samples. Either 3000 or 6000 cells per sample. As far as how many samples we will do in the future, I truly don't have a guess on that.
Thanks again.
For your data storage you should have reasonably fast disks (ideally a small RAID array of them) since your analysis will become input/output bound (CPU waiting for delivery of data to work on). Large SSD's get expensive fast so fast SATA disks would be ok.
Sounds like you do need to have Windows present which is fine. Are you going to use 10x or something else? 6 samples is not a lot.
Our samples are processed by 10X genomics, is that what you mean by 10x?
Our samples are processed by 10X genomics, is that what you mean by 10x?