I am using tophat2 for alignment which is showing "Error: failed to retrieve right read for pair # 243418 !". Then i used BBTools to repair seq data with - "repair.sh in1=broken1.fq in2=broken2 out1=fixed1.fq out2=fixed2.fq outs=singletons.fq repair" but still getting same issue.
How can i solve this issue?
Thanks in advance!!