Question: After trimming, how to proceed with the published tutorial of 16S analysis
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gravatar for rehab1171
22 days ago by
rehab11710
rehab11710 wrote:

I have trimmed all my reads. Now I wanted to continue with the tutorial file:///C:/Users/RehabElshehawy/Zotero/storage/Y9IA5GLM/tutorial.html I need to create: a collection of dataset reads. 1) I tried, It didn't work cause the names of the files were changed by mothur during trimming, hence they no longer contain R1 and R2. 2) So, I manually created the paired files and then I tried to create a list of theses paired file. It failed again and gave the error that a list can't be created from collections.

How do you guys proceed after trimming? Please explain it in simple steps, as I am a very beginner. Thank you in advance for your support

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ADD COMMENTlink modified 22 days ago • written 22 days ago by rehab11710
0
gravatar for vishwaas1704
22 days ago by
vishwaas170430
vishwaas170430 wrote:

Please read the manual. You can do everything with mother if you know what to do. Manual is quite an easy way to do that.

ADD COMMENTlink written 22 days ago by vishwaas170430

Which manual, can you please post it? I read the tutorial for the 16S workflow, and it does not include trimming before building contigs. I read the Quality control tutorial and it does not include how to proceed after trimming.

ADD REPLYlink written 22 days ago by rehab11710
0
gravatar for rehab1171
22 days ago by
rehab11710
rehab11710 wrote:

Which manual, can you please post it? I read the tutorial for the 16S workflow, and it does not include trimming before building contigs. I read the Quality control tutorial and it does not include how to proceed after trimming.

ADD COMMENTlink written 22 days ago by rehab11710
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