Can HOMER be used to predict the binding site of a transcription factor on a DNA sequence?
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2.4 years ago

I wish to find out if HOMER tool can be used to predict the binding site of a transcription factor on a list of DNA sequences.

Thank you in advance for your kind and helpful responses.

ChIP-Seq RNA-Seq genome gene HOMER • 1.1k views
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Ok thanks saw it. I wish to find out about the existence of a simple user-friendly online tool, or an R package rather than methods that are more computationally intensive.

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The suggested fimo approach is a bash one-liner on the command line. You won't find anything simpler :)

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Cross-posting the same question is generally discouraged. You could easily edit your Bioinformatics SE question to include these new parameters.

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2.4 years ago

You could run FIMO on your entire genome for TFs (transcription factors; DNA-binding proteins) of interest, which gives you binding sites: genomic intervals where those TFs bind.

https://bioinformatics.stackexchange.com/a/2491/776

You can then map or intersect promoter regions with those TF binding sites with BEDOPS bedmap or bedops, respectively:

https://bedops.readthedocs.io/en/latest/content/reference/statistics/bedmap.html

https://bedops.readthedocs.io/en/latest/content/reference/set-operations/bedops.html

The use of bedmap is recommended to assign TFs to a promoter of interest. This tool will return both the promoter region and any TFs that overlap it by the specified overlap criteria.

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